Journal: Virologica Sinica

Article Title: Modulation of the unfolded protein response by white spot syndrome virus via wsv406 targeting BiP to facilitate viral replication

doi: 10.1016/j.virs.2024.10.005

Figure Lengend Snippet: Wsv406 doesn't interfere the interaction of LvBiP with ATF6, IRE1 and PERK, but suppresses LvBiP ATPase activity. A His-tag pulldown for the detection of the interaction between BiP and ATF6, IRE1 or PERK by expression of wsv406 in a dose-dependent manner. The results were shown via western blotting. B Schematic representation of the procedures for measuring the ATPase activity of BiP. C ATPase activity of the recombinant BiP. GST-BiP were bound to Glutathione beads and MBP-wsv406 or MBP and then assessed for the ATPase activity. The values were normalized using the measure of empty beads.

Article Snippet: The following steps were the same as above, the primary antibodies used were rabbit anti-ATF4 antibody (Bioss, bs-1531R), rabbit anti-ATF6 antibody (Bioss, bs-1634R) and mouse anti-β-actin antibody (Sigma, A1978).

Techniques: Activity Assay, Expressing, Western Blot, Recombinant

Journal: Virologica Sinica

Article Title: Modulation of the unfolded protein response by white spot syndrome virus via wsv406 targeting BiP to facilitate viral replication

doi: 10.1016/j.virs.2024.10.005

Figure Lengend Snippet: Effects of wsv406 knockdown on ATF6 pathway during WSSV infection. A Transcription factor LvATF6 nuclear translocation in wsv406 silenced hemocytes infected by WSSV. The percentage of ATF6 nuclear translocation was calculated by Image J. The scale bar ​= ​5 ​μm. B–D Expression levels of LvASK1 ( B ), LvXBP1 ( C ) and WSSV genes ( D ) in hemocytes and gills of dsRNA-GFP treated or dsRNA-wsv406 treated shrimps at 48 ​h post WSSV infection. Differences were analyzed using Student's t -test ( ∗∗ P ​< ​0.01).

Article Snippet: The following steps were the same as above, the primary antibodies used were rabbit anti-ATF4 antibody (Bioss, bs-1531R), rabbit anti-ATF6 antibody (Bioss, bs-1634R) and mouse anti-β-actin antibody (Sigma, A1978).

Techniques: Knockdown, Infection, Translocation Assay, Expressing

Journal: Virologica Sinica

Article Title: Modulation of the unfolded protein response by white spot syndrome virus via wsv406 targeting BiP to facilitate viral replication

doi: 10.1016/j.virs.2024.10.005

Figure Lengend Snippet: The possible model illustrating that WSSV hijacks UPR signaling through wsv406 targeting BiP that facilitates to WSSV replication. In WSSV-infected shrimp, the viral protein wsv406 interacts with and inhibits the ATPase activity of the host protein BiP. This interaction leads to the activation of the PERK-eIF2α and ATF6 pathways, which subsequently promote the expression of other viral genes, facilitating WSSV replication.

Article Snippet: The following steps were the same as above, the primary antibodies used were rabbit anti-ATF4 antibody (Bioss, bs-1531R), rabbit anti-ATF6 antibody (Bioss, bs-1634R) and mouse anti-β-actin antibody (Sigma, A1978).

Techniques: Infection, Activity Assay, Activation Assay, Expressing